RAT SKELETAL MUSCLE β-GLUCURONIDASE LOCALIZATION BY POST-COUPLING TECHNIQUE USING NAPHTHOL AS-BI β-d-GLUCOSIDURONIC ACID AND FAST DARK BLUE R
نویسندگان
چکیده
منابع مشابه
Behaviour of the NO - P - D - Glucosiduronic Acid of N - Acetyl - N - phenylhydroxylamine as a Substrate for p - Glucuronidase
1. Biosynthetic sodium (N-acetyl-N-phenylhydroxylamine NO-f-D-glucosid)uronate is hydrolysed completely by purified mouse urinary fl-glucuronidase into the products N-acetyl-N-phenylhydroxylamine and glucuronic acid. The hydrolysis is inhibited by saccharo-(1 -*4)-lactone. These results not only confirm the identity and purity of the substrate but also establish it as a substrate for :-glucuron...
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(R)-1,1'-Bi(2-naphthol) ((R)-BINOL) in an acetonitrile solution lost optical activity upon irradiation with an Hg-Xe lamp. HPLC resolution of the product indicated that (R)-BINOL was racemized upon irradiation, and SEC analysis suggested that a polymeric product was formed in the course of racemization. It is proposed that polymerization of BINOL can occur before it is racemized and that a unit...
متن کاملPhenoxyacetohydrazide Schiff bases: β-glucuronidase inhibitors.
Phenoxyacetohydrazide Schiff base analogs 1-28 have been synthesized and their in vitro β-glucouoronidase inhibition potential studied. Compounds 1 (IC50=9.20±0.32 µM), 5 (IC50=9.47±0.16 µM), 7 (IC50=14.7±0.19 µM), 8 (IC50=15.4±1.56 µM), 11 (IC50=19.6±0.62 µM), 12 (IC50=30.7±1.49 µM), 15 (IC50=12.0±0.16 µM), 21 (IC50=13.7±0.40 µM) and 22 (IC50=22.0±0.14 µM) showed promising β-glucuronidase inhi...
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A continuous spectrophotometric assay has been developed for detecting β-glucuronidase activity. In the assay, Para-nitrophenyl β-D-glucuronide is cleaved to yield a chromophoric product. With the commercial E. coli enzyme, it is demonstrated that the reactions can be continuously monitored by the increase of absorbance at 405 nm. The method is highly sensitive and able to detect less than 1.4 ...
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Maintenance of skeletal muscle mass is contingent upon the dynamic equilibrium (fasted losses-fed gains) in protein turnover. Of all nutrients, the single amino acid leucine (Leu) possesses the most marked anabolic characteristics in acting as a trigger element for the initiation of protein synthesis. While the mechanisms by which Leu is 'sensed' have been the subject of great scrutiny, as a br...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1964
ISSN: 0022-1554,1551-5044
DOI: 10.1177/12.4.306-a